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mouse anti human socs2  (R&D Systems Hematology)
90
R&D Systems Hematology mouse anti human socs2
Figure 4. Hsv2-miR-H9-5p suppresses <t>SOCS2</t> expression by directly targeting the SOCS2 3′-UTR. (a) Heat map showed an intersection of mRNA microarray expression data and the target genes that TargetScan6.0 predicted. (b) Dual-luciferase assay of pmiR-GLO-SOCS2-3′-UTR reporter in cells into which Hsv2-miR-H9-5p had been transduced and predicted Hsv2-miR-H9- 5p target sequence in the 3′-UTR of SOCS2 (WT 3′-UTR) and mutant containing the mutated nucleotides in the SOCS2 3′-UTR (MUT 3′-UTR; *p < 0.05). (c) Real-time PCR analysis of SOCS2 after Hsv2-miR-h9-5p mimic and NC transfection at different times in LTEP-α-2 and SPC-α-1 cells. GAPDH was used as a loading control (*p < 0.05). (d) Western blot of SOCS2 after LTEP-α-2 cells were transfected with mimic or NC after 48 and 72 h. GAPDH served as the loading marker (*p < 0.05). (e) Western blot of SOCS2 after SPC-α-1 cells were transfected with mimic or NC after 48 and 72 h. GAPDH served as the loading marker (*p < 0.05). (f) Immunohistochemical staining data shows that SOCS2 is downregulated in the lung cancer tissues in comparison with normal adjacent tissues.
Mouse Anti Human Socs2, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+socs2/Human+SOCS-2+Antibody/pm28468588-75-28-32
Average 90 stars, based on 1 article reviews
mouse anti human socs2 - by Bioz Stars, 2026-09
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Figure 4. Hsv2-miR-H9-5p suppresses SOCS2 expression by directly targeting the SOCS2 3′-UTR. (a) Heat map showed an intersection of mRNA microarray expression data and the target genes that TargetScan6.0 predicted. (b) Dual-luciferase assay of pmiR-GLO-SOCS2-3′-UTR reporter in cells into which Hsv2-miR-H9-5p had been transduced and predicted Hsv2-miR-H9- 5p target sequence in the 3′-UTR of SOCS2 (WT 3′-UTR) and mutant containing the mutated nucleotides in the SOCS2 3′-UTR (MUT 3′-UTR; *p < 0.05). (c) Real-time PCR analysis of SOCS2 after Hsv2-miR-h9-5p mimic and NC transfection at different times in LTEP-α-2 and SPC-α-1 cells. GAPDH was used as a loading control (*p < 0.05). (d) Western blot of SOCS2 after LTEP-α-2 cells were transfected with mimic or NC after 48 and 72 h. GAPDH served as the loading marker (*p < 0.05). (e) Western blot of SOCS2 after SPC-α-1 cells were transfected with mimic or NC after 48 and 72 h. GAPDH served as the loading marker (*p < 0.05). (f) Immunohistochemical staining data shows that SOCS2 is downregulated in the lung cancer tissues in comparison with normal adjacent tissues.

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: A herpes simplex virus type 2-encoded microRNA promotes tumor cell metastasis by targeting suppressor of cytokine signaling 2 in lung cancer.

doi: 10.1177/1010428317701633

Figure Lengend Snippet: Figure 4. Hsv2-miR-H9-5p suppresses SOCS2 expression by directly targeting the SOCS2 3′-UTR. (a) Heat map showed an intersection of mRNA microarray expression data and the target genes that TargetScan6.0 predicted. (b) Dual-luciferase assay of pmiR-GLO-SOCS2-3′-UTR reporter in cells into which Hsv2-miR-H9-5p had been transduced and predicted Hsv2-miR-H9- 5p target sequence in the 3′-UTR of SOCS2 (WT 3′-UTR) and mutant containing the mutated nucleotides in the SOCS2 3′-UTR (MUT 3′-UTR; *p < 0.05). (c) Real-time PCR analysis of SOCS2 after Hsv2-miR-h9-5p mimic and NC transfection at different times in LTEP-α-2 and SPC-α-1 cells. GAPDH was used as a loading control (*p < 0.05). (d) Western blot of SOCS2 after LTEP-α-2 cells were transfected with mimic or NC after 48 and 72 h. GAPDH served as the loading marker (*p < 0.05). (e) Western blot of SOCS2 after SPC-α-1 cells were transfected with mimic or NC after 48 and 72 h. GAPDH served as the loading marker (*p < 0.05). (f) Immunohistochemical staining data shows that SOCS2 is downregulated in the lung cancer tissues in comparison with normal adjacent tissues.

Article Snippet: After blocking in 20 mmol/L Tris-HCl, pH 7.6, containing 150 mmol/L NaCl, 0.1% Tween 20, and 5% (wt/vol) nonfat dry milk, the blots were incubated with the specific mouse anti-human SOCS2 (MAB4979; R&D) and anti– glyceraldehyde 3-phosphate dehydrogenase (GAPDH; sc-365062; Santa Cruz Biotechnology, Inc.) overnight at 4°C.

Techniques: Expressing, Microarray, Luciferase, Sequencing, Mutagenesis, Real-time Polymerase Chain Reaction, Transfection, Control, Western Blot, Marker, Immunohistochemical staining, Staining, Comparison

Figure 5. Reconstitution of SOCS2 inverts the phenotypes generated by Hsv2-miR-H9-5p. (a) CCK-8 assay reveals that SOCS2 upregulation decreased growth of LTEP-α-2-mimic and SPC-α-1-mimic lung cancer cells (**p < 0.01). (b) Flow cytometric analysis of lung cancer cells transduced with SOCS2 or blank plasmid and Annexin V–APC/PI staining of cells treated with 20 µM cisplatin for 24 h (*p < 0.05). (c) Representative morphology of lung cancer cells transduced with SOCS2 and blank plasmid treated with 20 µM cisplatin for 24 h. (d) Reconstitution of SOCS2 reduced the cell migration of both LTEP-α-2-mimic and SPC-α-1-mimic cells (*p < 0.05). (e) Reinstallation of SOCS2 reduced the cell invasion of both LTEP-α-2-mimic and SPC-α-1-mimic cells (*p < 0.05).

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: A herpes simplex virus type 2-encoded microRNA promotes tumor cell metastasis by targeting suppressor of cytokine signaling 2 in lung cancer.

doi: 10.1177/1010428317701633

Figure Lengend Snippet: Figure 5. Reconstitution of SOCS2 inverts the phenotypes generated by Hsv2-miR-H9-5p. (a) CCK-8 assay reveals that SOCS2 upregulation decreased growth of LTEP-α-2-mimic and SPC-α-1-mimic lung cancer cells (**p < 0.01). (b) Flow cytometric analysis of lung cancer cells transduced with SOCS2 or blank plasmid and Annexin V–APC/PI staining of cells treated with 20 µM cisplatin for 24 h (*p < 0.05). (c) Representative morphology of lung cancer cells transduced with SOCS2 and blank plasmid treated with 20 µM cisplatin for 24 h. (d) Reconstitution of SOCS2 reduced the cell migration of both LTEP-α-2-mimic and SPC-α-1-mimic cells (*p < 0.05). (e) Reinstallation of SOCS2 reduced the cell invasion of both LTEP-α-2-mimic and SPC-α-1-mimic cells (*p < 0.05).

Article Snippet: After blocking in 20 mmol/L Tris-HCl, pH 7.6, containing 150 mmol/L NaCl, 0.1% Tween 20, and 5% (wt/vol) nonfat dry milk, the blots were incubated with the specific mouse anti-human SOCS2 (MAB4979; R&D) and anti– glyceraldehyde 3-phosphate dehydrogenase (GAPDH; sc-365062; Santa Cruz Biotechnology, Inc.) overnight at 4°C.

Techniques: Generated, CCK-8 Assay, Transduction, Plasmid Preparation, Staining, Migration